SIMON: Simple methods for analyzing DNA methylation by targeted bisulfite next-generation sequencing
نویسندگان
چکیده
منابع مشابه
Targeted DNA Methylation Analysis by Next-generation Sequencing
The role of epigenetic processes in the control of gene expression has been known for a number of years. DNA methylation at cytosine residues is of particular interest for epigenetic studies as it has been demonstrated to be both a long lasting and a dynamic regulator of gene expression. Efforts to examine epigenetic changes in health and disease have been hindered by the lack of high-throughpu...
متن کاملA classification approach for DNA methylation profiling with bisulfite next-generation sequencing data
MOTIVATION With the advent of high-throughput sequencing technology, bisulfite-sequencing-based DNA methylation profiling methods have emerged as the most promising approaches due to their single-base resolution and genome-wide coverage. However, statistical analysis methods for analyzing this type of methylation data are not well developed. Although the most widely used proportion-based estima...
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Recent scientific discoveries that resulted from the application of next-generation DNA sequencing technologies highlight the striking impact of these massively parallel platforms on genetics. These new methods have expanded previously focused readouts from a variety of DNA preparation protocols to a genome-wide scale and have fine-tuned their resolution to single base precision. The sequencing...
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In this review, we discuss the latest targeted enrichment methods and aspects of their utilization along with second-generation sequencing for complex genome analysis. In doing so, we provide an overview of issues involved in detecting genetic variation, for which targeted enrichment has become a powerful tool. We explain how targeted enrichment for next-generation sequencing has made great pro...
متن کاملThe Application of Next Generation Sequencing in DNA Methylation Analysis
DNA methylation is a major form of epigenetic modification and plays essential roles in physiology and disease processes. In the human genome, about 80% of cytosines in the 56 million CpG sites are methylated to 5-methylcytosines. The methylation pattern of DNA is highly variable among cells types and developmental stages and influenced by disease processes and genetic factors, which brings con...
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ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 2019
ISSN: 1342-4580,1347-6114
DOI: 10.5511/plantbiotechnology.19.0822a